Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF ameliorated HFFD-induced hepatic fibrosis progression in MASLD mice. (A and B) Representative images and quantification of hepatic Sirius scarlet staining. ( n = 3, scale bar = 50 μm). (C to E) Serum fibrosis factor HA, LN, and Col-IV levels. (F) Liver mRNA levels of α-SMA, Timp1, Tgf-β1, Col1a1, Fn1 , Pdgfrβ , and PAI-1 . (G and H) Western blot of α-SMA, TIMP1, and TGF-β1 protein expression and their quantification. Data were presented as the mean ± SEM ( n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the ND group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the HFFD group.

Article Snippet: LX2 cells or transfected LX2 cells were treated with 10 ng/ml TGF-β1 (HY-P7118, MCE) for 24 h [ ].

Techniques: Staining, Western Blot, Expressing

Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF alleviated MASLD fibrosis progression in vitro by inhibiting the SYK/SH3BP2 pathway. (A) LX2 cell survival ( n = 3). (B to G) Western blot of P-SYK, SYK, SH3BP2, α-SMA, TIMP1, and Tgf-β1 protein expression and their quantification. (H) mRNA levels of SYK, SH3BP2, α-SMA, TIMP1, Tgf-β1, Col1a1, FN1, Pdgfrβ , and PAI-1 . Data were presented as the mean ± SEM ( n = 4). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Tgf-β1 group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the Tgf-β1+oe-SYK group.

Article Snippet: LX2 cells or transfected LX2 cells were treated with 10 ng/ml TGF-β1 (HY-P7118, MCE) for 24 h [ ].

Techniques: In Vitro, Western Blot, Expressing

Journal: Science Advances

Article Title: TDRD3, a Tudor domain-containing protein, regulates Klf2 -dependent T reg differentiation and function to modulate immune tolerance

doi: 10.1126/sciadv.aea3960

Figure Lengend Snippet: ( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence of TGF-β (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).

Article Snippet: Recombinant TGF-β (#130-095-067) was from Miltenyi Biotec.

Techniques: Western Blot, Isolation, Labeling, Two Tailed Test